This method is generally time-consuming, requires more skill, necessary equipments, and is not as sensitive and reliable as the molecular methods. briefly, in HPV DNA detection by southern blot, the sample extracted DNA is digested by restriction enzymes and then runs in agarose gel electrophoresis to separate the digested DNA based on the size
Special tests can be performed on the Pap smear specimen to detect certain types (strains) of "high-risk" HPV that have a strong association with cervical cancer. However, just because this test is negative does not mean that a patient may not have been exposed to a low-risk strain of HPV. Note that there are over 100 types or strains of HPV
If colposcopy is negative for HC2-HPV-DNA on the initial examination, repeated HC2-HPV-DNA testing and cytology inspection at 12 months will be adequate to discern disease recurrence. We believe that persistent HPV infection is the most salient risk factor for the recurrence of cervical CIN, from which VAIN and vaginal cancer can develop.
Altogether, 1337 pieces of HPV-related DNA were detected, representing 23 new types of HPV, 10 established types of HPV and two known HPV DNA sequences. This new style of testing has highlighted
A different sample obtained with a sterile cytobrush was collected for DNA HR-HPV cobas® PCR Cell Collection Media test. This tool amplifies target DNA fragments by polymerase chain reaction and hybridization of nucleic acids for the detection of 14 HR-HPV types in a single analysis. The test specifically identifies HPV16 and HPV18 genotypes
Women with abnormal Pap test results or a positive HPV test (at least 1 pg of high-risk HPV DNA per milliliter) underwent colposcopy and biopsy, as did a random sample of women with negative tests. Sensitivity and specificity estimates were corrected for verification bias.
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    • hpv dna test negative means